12497 Dnajb8 Status: Cancelled
| Gene | Dnajb8 |
| Chromosome | 6 |
| Deletion Start | 88,222,487 |
| Deletion End | 88,223,242 |
| Deletion Size | 756 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,222,629 | 88,222,648 | TTTCCGAAGCCTACGAAGTC |
| TUP | 88,222,658 | 88,222,684 | TCTAAGAAGCGTTCTGTGTACGACCGG |
| TUR | 88,222,696 | 88,222,714 | GCCACCTGCTCTCCATCTG |
| TDF | 88,222,976 | 88,222,994 | GCTTCGTTTGGTGGCTCTG |
| TDP | 88,223,000 | 88,223,027 | TCAGGGTTCAAGTCAGTGATGTCATCGA |
| TDR | 88,223,053 | 88,223,074 | CTCAATGATTCGCTTGGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | TCCCAGGGAGTCAAACAAAC |
| SD | GGTGGAGATGAGGTCAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
