Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12503 Fzd4 Status: ES cell colonies screened / QC positives

Gene	Fzd4
Chromosome	7
Deletion Start	89,404,690
Deletion End	89,408,432
Deletion Size	3,743
Genome Build	38

MGI ID	MGI:108520
NCBI Gene ID	14366
BAC ID	129I14
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	89,404,849	89,404,868	AACCTCGGCTACAACGTGAC
TUP	89,404,869	89,404,895	CAAGATGCCCAACTTAGTGGGACACGA
TUR	89,404,928	89,404,945	GGATGAGCGGCGTGAAAG
TDF	89,408,341	89,408,363	GCAACGAGACTGTGGTATAAGAC
TDP	89,408,367	89,408,391	CCGGCTTCCTCGTTCCTCATTGTGA
TDR	89,408,397	89,408,419	CAAACTGAGATTCCCTGCATCAC

PCR Primers

Primer	Sequence
SU	CTTACAGCAGGAGAGGAGCC
SD	AACTCTGCAACACGTCTCCC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	97 bp	+	+	-
TDF	TDR	79 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12503A-A6	VGB6	C57BL/6NTac
12503A-E5	VGB6	C57BL/6NTac