12510 Cacng2 Status: Cancelled
| Gene | Cacng2 |
| Chromosome | 15 |
| Deletion Start | 78,118,888 |
| Deletion End | 78,118,688 |
| Deletion Size | 201 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,118,861 | 78,118,881 | TTGATCGAGGTGTTCAAATGC |
| TUP | 78,118,836 | 78,118,859 | TTTAACCACCGTTGGTGCTTTCGC |
| TUR | 78,118,792 | 78,118,812 | GCCAATAGTCGGTTCCCACAG |
| TDF | 78,118,751 | 78,118,773 | GCAAGACCAAAAGTGTCAGTGAG |
| TDP | 78,118,714 | 78,118,743 | CCAGCAAGAAGAACGAGGAAGTTATGACCC |
| TDR | 78,118,687 | 78,118,708 | GGCAGCAGGTTCTCCATAATCC |
PCR Primers
| Primer | Sequence |
| SU | CATATGGAAACTGGAGACCAG |
| SD | AAGCCAAAGAGTAGAGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
