| Gene | Krt5 |
| Chromosome | 15 |
| Deletion Start | 101,712,807 |
| Deletion End | 101,707,374 |
| Deletion Size | 5,434 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,711,876 | 101,711,895 | AGGAGCATCAGTGCCGATTG |
| TUP | 101,711,852 | 101,711,874 | CCCTCACAGGTCACAGGCCAGGT |
| TUR | 101,711,810 | 101,711,830 | TGCTACCTGTCACATGCTGTC |
| TDF | 101,707,915 | 101,707,938 | CCTTACCTCTACCAAGTACCTGAC |
| TDP | 101,707,893 | 101,707,914 | TCCCTGCCTTGCCTCTTGGGTC |
| TDR | 101,707,829 | 101,707,852 | CAAAGTAGCAGAATGGGCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCCTGGCATCCTTCTAGG |
| SD | AAGAAACCAAGCACACCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12513A-A2 | VGB6 | C57BL/6NTac | |
| 12513A-A6 | VGB6 | C57BL/6NTac | |
| 12513A-B1 | VGB6 | C57BL/6NTac | |
| 12513A-H5 | VGB6 | C57BL/6NTac | |
