12515 Ap3s1 Status: ES cell colonies screened / QC one positive
| Gene | Ap3s1 |
| Chromosome | 18 |
| Deletion Start | 46,742,016 |
| Deletion End | 46,758,109 |
| Deletion Size | 16,094 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,742,530 | 46,742,550 | CCAGTCTGTCCAGGGTGTTTC |
| TUP | 46,742,554 | 46,742,575 | ACGGAAGCATCGGAAGCCCAGT |
| TUR | 46,742,578 | 46,742,597 | TCAATAGCCACCGGGAAGTC |
| TDF | 46,757,412 | 46,757,436 | CTGTGACTATGGAGTTGTGATCTTG |
| TDP | 46,757,438 | 46,757,462 | AAGCACTTGCACATGCCCTGTGATC |
| TDR | 46,757,465 | 46,757,485 | GGCTAAGCAACAGGCAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGGTAGATAGTATGTTGG |
| SD | TGAGGACAGCAGCTTTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
