| Gene | Gpr101 |
| Chromosome | X |
| Deletion Start | 57,501,785 |
| Deletion End | 57,500,159 |
| Deletion Size | 1,627 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,501,376 | 57,501,398 | CTGACCATCATCCACCCTCTTTC |
| TUP | 57,501,349 | 57,501,373 | ACCCATCCAAGATGACCAACCGACG |
| TUR | 57,501,313 | 57,501,333 | GCTGCAATCCAGGTGCCATAG |
| TDF | 57,500,784 | 57,500,803 | CTAGCAGCGCAAAGGCAGAC |
| TDP | 57,500,761 | 57,500,782 | CCGGCCGCATAGATGCCAATCA |
| TDR | 57,500,717 | 57,500,741 | CCATGCCAAACTCTACATCATCTTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGGAACAGAGTGCAAGTG |
| SD | TAGCCCATGAGTTCCTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12517B-D2 | VGB6 | C57BL/6NTac | |
| 12517B-E6 | VGB6 | C57BL/6NTac | |
