12518 Efnb1 Status: ES cell colonies screened / QC no positives
| Gene | Efnb1 |
| Chromosome | X |
| Deletion Start | 99,136,889 |
| Deletion End | 99,147,569 |
| Deletion Size | 10,681 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,137,200 | 99,137,219 | TAGCTGCGTTTCGATCCTTC |
| TUP | 99,137,222 | 99,137,245 | TTTGGCCCTACTCTCAAGTCCGGA |
| TUR | 99,137,258 | 99,137,282 | ACATTCTATATCTGCCTGGTGTGAC |
| TDF | 99,146,682 | 99,146,703 | CACCTAAGGGAGTTGAGGACAC |
| TDP | 99,146,704 | 99,146,726 | CCATGCTGGCAGAGTCCTCCCTC |
| TDR | 99,146,731 | 99,146,750 | TGAGGGCCAAGAGAAGACAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGAGGACTTGCAGTCTC |
| SD | CTCAGAAAGCCTGGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
