12534 C1qtnf5 Status: ES cell clone microinjected
| Gene | C1qtnf5 |
| Chromosome | 9 |
| Deletion Start | 43,915,844 |
| Deletion End | 43,916,933 |
| Deletion Size | 1,090 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,916,239 | 43,916,261 | CAGAAACCCTGATGCCTCTACTC |
| TUP | 43,916,280 | 43,916,302 | CCCACAGGAGGCAATAGCCAGGG |
| TUR | 43,916,337 | 43,916,359 | GGAGAAATTAGGAGCCGCAGAAG |
| TDF | 43,916,790 | 43,916,809 | GGGCGTGGGTGATTACATTG |
| TDP | 43,916,813 | 43,916,841 | TCTATGCCAGCATCAAGACAGACAGTACC |
| TDR | 43,916,859 | 43,916,880 | GGAGCTGTGCCAGTCAGAATAG |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGAAGCCATTCAAAGC |
| SD | GGAGCAGCAGAGATGGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12534A-D8 | VGB6 | C57BL/6NTac | |
| 12534A-G8 | VGB6 | C57BL/6NTac | |
| 12534A-H4 | VGB6 | C57BL/6NTac | |
| 12534A-H11 | VGB6 | C57BL/6NTac | |
