| Gene | Camta1 |
| Chromosome | 4 |
| Deletion Start | 151,861,666 |
| Deletion End | 151,830,426 |
| Deletion Size | 31,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,860,739 | 151,860,758 | CCCTGTGCAGCTCTGAAGTC |
| TUP | 151,860,705 | 151,860,732 | AAATAGGCAGGCAGACACTTCTTCCAGA |
| TUR | 151,860,674 | 151,860,696 | AACCCAGGAAAGTGTACGGAAAG |
| TDF | 151,830,720 | 151,830,739 | TGAAGCTCAGGCTGTTTGTG |
| TDP | 151,830,697 | 151,830,715 | TCACAGGCAGGCGCTTGCT |
| TDR | 151,830,655 | 151,830,675 | TCGCTCCTCTAAGTTCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | TGTCAGCCTCATCCTGATTG |
| SD | CTGTTCTCTGTCTCACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12535A-B3 | VGB6 | C57BL/6NTac | |
| 12535B-A6 | VGB6 | C57BL/6NTac | |
| 12535B-C6 | VGB6 | C57BL/6NTac | |
| 12535B-H8 | VGB6 | C57BL/6NTac | |
