12536 Gpr15 Status: Design Not Possible
| Gene | Gpr15 |
| Chromosome | 16 |
| Deletion Start | 58,718,721 |
| Deletion End | 58,717,572 |
| Deletion Size | 1,150 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,718,664 | 58,718,685 | GACTACACAAGCCCAGATCCTC |
| TUP | 58,718,640 | 58,718,662 | TTTCCTGGAGACTCCCTCCCACC |
| TUR | 58,718,578 | 58,718,601 | CACCACTCCAGTCAAGAATACAAC |
| TDF | 58,717,764 | 58,717,782 | CGTTGTCTGTGCCCTTGTC |
| TDP | 58,717,740 | 58,717,763 | TGAAGACCCACAACTTTGGGAGCA |
| TDR | 58,717,692 | 58,717,716 | TGAAGTTGGAAAGAGCCTTAGTGAG |
PCR Primers
| Primer | Sequence |
| SU | TACCAATCACAGGGTGCTTC |
| SD | GTACAAACAGCGGATGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
