12542 Apln Status: ES cell colonies screened / QC positives
| Gene | Apln |
| Chromosome | X |
| Deletion Start | 45,387,751 |
| Deletion End | 45,381,479 |
| Deletion Size | 6,273 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,387,109 | 45,387,129 | GGGCTCAAGCTACTGGAGATG |
| TUP | 45,387,079 | 45,387,107 | TGTGCCAACATGTAGACGTGACACTTGTT |
| TUR | 45,387,058 | 45,387,077 | GCAGCGTGCTCCTGACTATG |
| TDF | 45,381,619 | 45,381,640 | CCACTGATGTTGCCTCCAGATG |
| TDP | 45,381,590 | 45,381,617 | AACAGGACTAGAAGAAGGAAGCATGCGC |
| TDR | 45,381,563 | 45,381,583 | CCTCGAAGTTCTGGGCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | CACATGACCAAGTGGGCTC |
| SD | AAGCTCTCCTGCTCCCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12542A-C1 | VGB6 | C57BL/6NTac | |
| 12542A-C8 | VGB6 | C57BL/6NTac | |
| 12542A-D3 | VGB6 | C57BL/6NTac | |
| 12542A-F10 | VGB6 | C57BL/6NTac | |
| 12542A-G11 | VGB6 | C57BL/6NTac | |
