12554 Mbtps2 Status: ES cell colonies screened / QC no positives
| Gene | Mbtps2 |
| Chromosome | X |
| Deletion Start | 157,598,567 |
| Deletion End | 157,561,389 |
| Deletion Size | 37,179 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 157,598,107 | 157,598,127 | GCTAAGGTTAGGACCCGTGAG |
| TUP | 157,598,076 | 157,598,105 | CGCTGCTAGTGTTGTGATAAACCGAAATCA |
| TUR | 157,598,031 | 157,598,053 | TCCTGTGTACTTTCGAGTTTGAC |
| TDF | 157,561,648 | 157,561,671 | GAAGGCAGATACATAGTGAGCATC |
| TDP | 157,561,618 | 157,561,643 | TGCTGCAATAAGTAGATCCCTGTGCC |
| TDR | 157,561,572 | 157,561,592 | CCACATGGGCATCCTGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCGGAGATGGAAATCAGTG |
| SD | GATGTCTTAGTGGTTTAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
