12577 Scube1 Status: Cancelled
| Gene | Scube1 |
| Chromosome | 15 |
| Deletion Start | 83,724,903 |
| Deletion End | 83,703,422 |
| Deletion Size | 21,482 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,724,415 | 83,724,433 | CGAGGCGTAGTGGGAACAG |
| TUP | 83,724,388 | 83,724,411 | AAAGGGCCCAGGAAGAAGCCCTCA |
| TUR | 83,724,295 | 83,724,318 | CCTTTGATCTGCCTTTCTCCATTC |
| TDF | 83,704,268 | 83,704,285 | TGGCAGTGTTGGCAAGTC |
| TDP | 83,704,241 | 83,704,266 | TTAGAAGGAGCCAGAGCCAGACTTGA |
| TDR | 83,704,220 | 83,704,240 | CCACAGCGAAGCAGGTAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGAAGGGAAACAGACACC |
| SD | CTCAAAACTGAGCCACATCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 139 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
