12578 Zfp933 Status: Cancelled
| Gene | Zfp933 |
| Chromosome | 4 |
| Deletion Start | 147,848,277 |
| Deletion End | 147,825,592 |
| Deletion Size | 22,686 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,847,621 | 147,847,643 | GCTAGGCCATTCCCATTGATAAG |
| TUP | 147,847,591 | 147,847,616 | TTGTTCTCTGCTGCTCAAATGAGCCA |
| TUR | 147,847,540 | 147,847,560 | GAGCGGCTTCCCAATAAACTG |
| TDF | 147,825,740 | 147,825,759 | CGCACACTGGAGAGAAACAC |
| TDP | 147,825,717 | 147,825,738 | TTGAATAAGCAAAGCAATATGG |
| TDR | 147,825,691 | 147,825,713 | GGCTACTGCTATATGCAAAGGCT |
PCR Primers
| Primer | Sequence |
| SU | TCACGTGTTACAGCGGTTTC |
| SD | TGCAACCAAAATAGTGACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
