12584 C1qtnf4 Status: ES cell colonies screened / QC no positives
| Gene | C1qtnf4 |
| Chromosome | 2 |
| Deletion Start | 90,889,751 |
| Deletion End | 90,890,366 |
| Deletion Size | 616 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCCGTTGGCCTTCGACA |
| TUP | 90,889,992 | 90,890,016 | CCGAGCTGGTAAACATAGGTGGCGA |
| TUR | | | AGGCGGCAGCGGAACAC |
| TDF | 90,890,241 | 90,890,261 | GCCACGATCACGATGGCTATG |
| TDP | 90,890,263 | 90,890,286 | CGCCTACAGCAACCACGGCAAGTA |
| TDR | 90,890,292 | 90,890,312 | ACACCAGGAAGCCTGAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGCTACTGTCCAACCCTC |
| SD | GAGGGATGCACTGGACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
