12589 Lrrc4b Status: ES cell colonies screened / QC no positives
| Gene | Lrrc4b |
| Chromosome | 7 |
| Deletion Start | 44,444,913 |
| Deletion End | 44,462,909 |
| Deletion Size | 17,997 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,445,180 | 44,445,200 | CGCTACCTGAACCTGCAAGAG |
| TUP | 44,445,202 | 44,445,224 | ACAGCATCCAGGTGAGCCCGCCT |
| TUR | 44,445,246 | 44,445,264 | GCACCCTTTGTGCCTTGAC |
| TDF | 44,461,920 | 44,461,939 | CATGACGCACGGTTCCTATC |
| TDP | 44,461,941 | 44,461,963 | TGTGCGCATTTCAGTGCTGCACG |
| TDR | 44,461,972 | 44,461,993 | CGGTGACATTGGTGAAGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AAACAAAGGCCTTGAGCATC |
| SD | AGTGATTGACGCAAACCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
