12592 Cd84 Status: Cancelled
| Gene | Cd84 |
| Chromosome | 1 |
| Deletion Start | 171,851,844 |
| Deletion End | 171,886,466 |
| Deletion Size | 34,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 171,852,060 | 171,852,083 | GCAGGAACTTACAAAGCAGACATC |
| TUP | 171,852,086 | 171,852,110 | TGAAGAGAATGAGGAAACCATCACC |
| TUR | 171,852,136 | 171,852,155 | CCCGTGCTGCCATAACTTAC |
| TDF | 171,885,536 | 171,885,556 | GCACTCTGGCTGAGGAGAAAG |
| TDP | 171,885,565 | 171,885,594 | TTCCAGATGCTGTCCTGTAAGAAAGATCCG |
| TDR | 171,885,597 | 171,885,620 | TGCACTGAGGAATAAATGGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | AAGTTTTGGTGGTCATAGCAC |
| SD | TTGCATCAGGGTTCCTTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
