| Gene | Crym |
| Chromosome | 7 |
| Deletion Start | 120,201,921 |
| Deletion End | 120,186,610 |
| Deletion Size | 15,312 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,201,560 | 120,201,581 | GCCACCTGCTGTAATTCAACTC |
| TUP | 120,201,535 | 120,201,559 | CCTGTCCTTGGAAAGGGAGGTACCA |
| TUR | 120,201,487 | 120,201,505 | CCGCAAATCGCTCTACCTC |
| TDF | 120,187,302 | 120,187,321 | TGGCTGGGCCTGTAATTCTG |
| TDP | 120,187,272 | 120,187,300 | TGTTATGGAGGTGGAAACATGGAGATCCC |
| TDR | 120,187,228 | 120,187,247 | GTGCTGAGCTTGCTGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGAGCAAGGTGGAACCAG |
| SD | TCCTCTAGGGATTGAGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12596B-A8 | VGB6 | C57BL/6NTac | |
| 12596B-F4 | VGB6 | C57BL/6NTac | |
| 12596B-D4 | VGB6 | C57BL/6NTac | |
| 12596C-C6 | VGB6 | C57BL/6NTac | |
