12602 St8sia3 Status: ES cell colonies screened / QC positives
| Gene | St8sia3 |
| Chromosome | 18 |
| Deletion Start | 64,425,129 |
| Deletion End | 64,431,539 |
| Deletion Size | 6,411 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,425,611 | 64,425,633 | AGAGATGGACAAGGCTTGAGGTG |
| TUP | 64,425,634 | 64,425,656 | AGCGACCTGGCTGAGCACTAGCG |
| TUR | 64,425,703 | 64,425,722 | GAAGGGATGCGAGAGCAGTC |
| TDF | 64,431,021 | 64,431,040 | CGCATACTGAAGCTGGGAAG |
| TDP | 64,431,041 | 64,431,068 | TGTATCTAGCGGTGAAACCCAAACAGTC |
| TDR | 64,431,082 | 64,431,103 | AGTCAAGAAGCAGGGCAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | CCATCTTCCATTTCAGCTCC |
| SD | CAGAGCTCCCTGGTTGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12602A-B3 | VGB6 | C57BL/6NTac | |
| 12602A-F1 | VGB6 | C57BL/6NTac | |
| 12602A-F4 | VGB6 | C57BL/6NTac | |
