12603 D1Bwg0212e Status: Cancelled
| Gene | D1Bwg0212e |
| Chromosome | 1 |
| Deletion Start | 39,535,842 |
| Deletion End | 39,545,327 |
| Deletion Size | 9,486 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,536,698 | 39,536,719 | AAGCCTCCGAGTAAAGAAGTCG |
| TUP | 39,536,730 | 39,536,756 | CCTGAGGTTGAAGTCCACATCACCACA |
| TUR | 39,536,768 | 39,536,787 | CCAAGGAGTTGGCTGCAAAG |
| TDF | 39,544,620 | 39,544,641 | GGAGGGAGACCATGATTGACAG |
| TDP | 39,544,642 | 39,544,663 | TGATCCAGCCAGGCACGCTCAG |
| TDR | 39,544,726 | 39,544,747 | GGCACTTAACCTCTGATGCTTC |
PCR Primers
| Primer | Sequence |
| SU | AACGGCGGTCTCTACATCAC |
| SD | TCACCGTAAATTCTCAGCGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
