12607 Tuft1 Status: ES cell colonies screened / QC no positives
| Gene | Tuft1 |
| Chromosome | 3 |
| Deletion Start | 94,658,781 |
| Deletion End | 94,628,202 |
| Deletion Size | 30,580 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,658,216 | 94,658,238 | CGGGATAGGTCTCAGACAACTAC |
| TUP | 94,658,180 | 94,658,206 | ACCAGATTCTACCTTTCGCCCAGAGGA |
| TUR | 94,658,155 | 94,658,172 | ACCGTCGAGCGGAGAGCT |
| TDF | 94,628,325 | 94,628,344 | CAGCTTTCCCTTCCCGTCTC |
| TDP | 94,628,301 | 94,628,324 | TGCTCCTGTTCTAGGTGGACCCTA |
| TDR | 94,628,253 | 94,628,272 | GCAACTCCTGCACAGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCAACTCAGACGACCAGACC |
| SD | AACTATCCTCGACCTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
