| Gene | Leprel2 |
| Chromosome | 6 |
| Deletion Start | 124,857,684 |
| Deletion End | 124,841,661 |
| Deletion Size | 16,024 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,856,972 | 124,856,995 | TGGAGAACGTGTCAAATTGGAATG |
| TUP | 124,856,932 | 124,856,959 | AGTAGACCGAATATGTTGGGAGGGCTCC |
| TUR | 124,856,868 | 124,856,892 | TCCATCTGCTCTTCGTATCTGAAAG |
| TDF | 124,842,413 | 124,842,431 | TGGAGTCAGGCAGGAAAGG |
| TDP | 124,842,386 | 124,842,412 | TCAGGCTCTAGGATAGGGAACCAACAA |
| TDR | 124,842,342 | 124,842,362 | CCGAACATGGAAGCCAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CGTGGGTAGATGAACGTGTG |
| SD | CTCTGCACTCCTCCACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12611A-B3 | VGB6 | C57BL/6NTac | |
| 12611A-B7 | VGB6 | C57BL/6NTac | |
| 12611A-F5 | VGB6 | C57BL/6NTac | |
| 12611A-E10 | VGB6 | C57BL/6NTac | |
