12612   Auh     Status: ES cell colonies screened / QC one positive

GeneAuh
Chromosome13
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1338011
NCBI Gene ID11992
BAC ID333C17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF52,929,28052,929,298GGGCCAGGGTTGCTTCTTG
TUP52,929,25452,929,278TGTGTAATAGCTCCCTGCTACCCCA
TUR52,929,23052,929,250TCCTACAACTCTCGCACTGAC
TDF52,899,18152,899,204TCATAATTGCGACCTCATCCCTTG
TDP52,899,15352,899,180CCTTTAAACCAGCGCTTCTCAGGTTGGA
TDR52,899,11852,899,140GCCAACTACAACAAAGTCACCAC

PCR Primers

PrimerSequence
SUGTATCTTCCTTCCTTCCGGG
SDCTACCTGACATATTTCACGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR87 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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