12617 Psg16 Status: ES cell colonies screened / QC no positives
| Gene | Psg16 |
| Chromosome | 7 |
| Deletion Start | 17,090,569 |
| Deletion End | 17,098,227 |
| Deletion Size | 7,659 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,091,011 | 17,091,035 | GGAAGATTGGGTTTGAATGTGGAAG |
| TUP | 17,091,044 | 17,091,071 | TCCTCATGGAAGTGAGCATCAGGAAGCC |
| TUR | 17,091,084 | 17,091,106 | TCAGGAGATGAGTCAGGACAGAG |
| TDF | 17,097,973 | 17,097,994 | GCTGTCTGAAGGATGACTTTGG |
| TDP | 17,097,997 | 17,098,024 | CCCTCTTCTGTTTCTTTGCAGAGCCTGT |
| TDR | 17,098,040 | 17,098,061 | AACTGTGCTGTCTGTGACTCTC |
PCR Primers
| Primer | Sequence |
| SU | GTGAAGCTAGCTGAGAGGGC |
| SD | GCCTGTTCTTCTACACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
