12619 B3galt1 Status: ES cell colonies screened / QC positives
| Gene | B3galt1 |
| Chromosome | 2 |
| Deletion Start | 67,956,003 |
| Deletion End | 67,957,041 |
| Deletion Size | 1,039 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ACTTGAGCATAACCAGACCTACTTC |
| TUP | | | ATCCTACACTGGCTCCAAGCCGTTCA |
| TUR | | | CCTGGCAACTGTTAGGTGAC |
| TDF | | | CGCAGTAAGTGGTATATGCCTAGAG |
| TDP | | | TGTACCCTGACAGCAACTACCCACC |
| TDR | | | AGCCAGTCCCTGAACAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGAAGATTTCTGAGGCATGG |
| SD | AGTGTGTGTTCCTGACTGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
