12623 Prl2c5 Status: ES cell colonies screened / QC no positives
| Gene | Prl2c5 |
| Chromosome | 13 |
| Deletion Start | 13,182,785 |
| Deletion End | 13,191,820 |
| Deletion Size | 9,036 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,182,961 | 13,182,985 | CAGTGGATCTGAGAAAACCTTTCTG |
| TUP | 13,182,997 | 13,183,024 | TGGAGCAAATGCACAGTGCATTTATGGA |
| TUR | 13,183,130 | 13,183,160 | CAAACATCATGATTTCTCATACAACACATTG |
| TDF | 13,191,461 | 13,191,488 | CTGATCAGACAAAAAAGACAAGCTACAC |
| TDP | 13,191,490 | 13,191,509 | TTCTTCTCTTTTAGCTACCC |
| TDR | 13,191,510 | 13,191,534 | GTATGGATTTCCATGATGTTGGTGA |
PCR Primers
| Primer | Sequence |
| SU | ATGAACATGAGATGAACCACG |
| SD | AAAACACCAGTTCTTGATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 200 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
