| Gene | Trim35 |
| Chromosome | 14 |
| Deletion Start | 66,297,073 |
| Deletion End | 66,309,433 |
| Deletion Size | 12,361 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,297,443 | 66,297,462 | TCTTCTGCCTGGAGGACAAG |
| TUP | 66,297,464 | 66,297,486 | AGCTGCTGTGCTGTGCTTGCCAG |
| TUR | 66,297,508 | 66,297,526 | TGATGGGCTGCACACGATG |
| TDF | 66,308,760 | 66,308,779 | AGCTGCTGGCTGGCTCAAAG |
| TDP | 66,308,780 | 66,308,803 | TGGCTGATGACCTCACGAGTGTCA |
| TDR | 66,308,824 | 66,308,844 | GCGCTCTGGATTCTCCACTTG |
PCR Primers
| Primer | Sequence |
| SU | GGGAGCTCAGGAGTTAAGCC |
| SD | CCCTACAGTGTTCCCACAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12629A-H9 | VGB6 | C57BL/6NTac | |
| 12629A-D7 | VGB6 | C57BL/6NTac | |
| 12629A-D12 | VGB6 | C57BL/6NTac | |
| 12629A-H3 | VGB6 | C57BL/6NTac | |
