12630 Noa1 Status: ES cell colonies screened / QC no positives
| Gene | Noa1 |
| Chromosome | 5 |
| Deletion Start | 77,310,053 |
| Deletion End | 77,294,337 |
| Deletion Size | 15,717 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,309,867 | 77,309,887 | CGACCGAGGACTATGCAGAAG |
| TUP | 77,309,842 | 77,309,864 | CCGGACACGGAGGAACGCTTTCT |
| TUR | 77,309,789 | 77,309,807 | GCAACTCCCGCACTTGTTC |
| TDF | 77,295,098 | 77,295,117 | CGAGCCAGTTTCCCTTTCTC |
| TDP | 77,295,071 | 77,295,094 | CGCACGTCTCCCTGGAGGACTAGT |
| TDR | 77,294,993 | 77,295,012 | GCCAGTCACTGGATGGTATC |
PCR Primers
| Primer | Sequence |
| SU | TGAAAGTTTCTTGCTGCGTG |
| SD | CTGGATTGCAATGTCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
