12644 4921506M07Rik Status: ES cell colonies screened / QC no positives
| Gene | 4921506M07Rik |
| Chromosome | 12 |
| Deletion Start | 57,575,820 |
| Deletion End | 57,576,680 |
| Deletion Size | 861 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,575,916 | 57,575,936 | GAGCAACTCAGGCAGAGACTG |
| TUP | 57,575,941 | 57,575,963 | CCAGGCCGACTTTGAAGGCACGT |
| TUR | 57,575,967 | 57,575,989 | CGTTCTTCCTTCTCCTGGTCTTC |
| TDF | 57,576,441 | 57,576,461 | CTGCGGATTCGTACCCACTTC |
| TDP | 57,576,463 | 57,576,484 | AGCGCGATCTCGTGCGATGGCA |
| TDR | 57,576,486 | 57,576,506 | GGATCTGGATGAGGGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCACAACATTGCAGTCTAAAGC |
| SD | ACCTCTCCATTTCTCTGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
