12645 Nt5c1b Status: Cancelled
| Gene | Nt5c1b |
| Chromosome | 12 |
| Deletion Start | 10,370,066 |
| Deletion End | 10,390,158 |
| Deletion Size | 20,093 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,370,421 | 10,370,442 | TGGAGTGAGCTGAGTTTTGGAG |
| TUP | 10,370,459 | 10,370,487 | TGGCTTACAAAAGGTTACATGGGTGTTGA |
| TUR | 10,370,493 | 10,370,515 | GAAGCCCCACTACTTCCTATTTC |
| TDF | 10,389,910 | 10,389,930 | GTAGTGCAGCGAGTTCAGGTG |
| TDP | 10,389,931 | 10,389,953 | CCCGTGTGCTGAAGACCCTTCGC |
| TDR | 10,390,037 | 10,390,059 | GGAACATTTGGTCGTCGAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGACAAAGCCAAGGAGAAC |
| SD | TTGATCAGACTCATTCCACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
