12655 1700108M19Rik Status: ES cell colonies screened / QC positives
| Gene | 1700108M19Rik |
| Chromosome | 12 |
| Deletion Start | 36,939,305 |
| Deletion End | 36,935,126 |
| Deletion Size | 4,180 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,938,376 | 36,938,395 | GTATGCTGCCAGGCACAAAG |
| TUP | 36,938,355 | 36,938,375 | TGCTGGCCTAGCATGCCCAAA |
| TUR | 36,938,334 | 36,938,354 | AGTGCTGGAGATCAAACCAAC |
| TDF | 36,935,250 | 36,935,273 | CCCAGAAGATGCTGTGTTTGTAAG |
| TDP | 36,935,221 | 36,935,248 | TCCACGAAGAGATCCCTGATGACGTTCA |
| TDR | 36,935,198 | 36,935,219 | AACGGTGTTCCAGTTCATGCTG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGCCACTATGACAGGAGG |
| SD | TATACGTGGGTAACTCATATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12655A-B7 | VGB6 | C57BL/6NTac | |
| 12655A-D4 | VGB6 | C57BL/6NTac | |
| 12655A-G1 | VGB6 | C57BL/6NTac | |
| 12655A-G11 | VGB6 | C57BL/6NTac | |
| 12655A-H6 | VGB6 | C57BL/6NTac | |
| 12655A-C3 | VGB6 | C57BL/6NTac | |
