12656 Neurl4 Status: ES cell colonies screened / QC no positives
| Gene | Neurl4 |
| Chromosome | 11 |
| Deletion Start | 69,901,901 |
| Deletion End | 69,913,371 |
| Deletion Size | 11,471 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,902,274 | 69,902,292 | TCTCCAACCGAGCCTCCAG |
| TUP | 69,902,294 | 69,902,314 | TACCGATAGCGAGCCGGCACA |
| TUR | 69,902,330 | 69,902,354 | CTCCGTGTCCTTCTCCTTTCTCTTC |
| TDF | 69,913,253 | 69,913,271 | CAGCCCATCGGAACTTGAG |
| TDP | 69,913,277 | 69,913,302 | CACTAAGGAGAAGGGAGCCACACTCC |
| TDR | 69,913,304 | 69,913,322 | TCGTACCAGCAGGGCATAG |
PCR Primers
| Primer | Sequence |
| SU | GTCGCAAAGTGTATCTGGGC |
| SD | TCATGTGGGCACAGAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
