12657 Ppp1r42 Status: ES cell colonies screened / QC no positives
| Gene | Ppp1r42 |
| Chromosome | 1 |
| Deletion Start | 10,003,360 |
| Deletion End | 9,968,770 |
| Deletion Size | 34,591 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,002,478 | 10,002,500 | CCATGCCCAACTAAAACCCATAA |
| TUP | 10,002,448 | 10,002,474 | TTCTGTCTTAGCCTCCAAGTACCACCA |
| TUR | 10,002,424 | 10,002,446 | CAGGAATGGTATAGAGGCTGCTT |
| TDF | 9,969,667 | 9,969,686 | TGGCTTGATCTCGGCCTTAG |
| TDP | 9,969,632 | 9,969,661 | TACTAGGTATTCAACACTGGACAAGTGGCC |
| TDR | 9,969,611 | 9,969,631 | GAGGATGGGATTCCGGGATTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGGCTACAGCCAGATCC |
| SD | TTCTAACTTTCCACGTCGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
