12660 Treml4 Status: Cancelled
| Gene | Treml4 |
| Chromosome | 17 |
| Deletion Start | 48,264,383 |
| Deletion End | 48,274,592 |
| Deletion Size | 10,210 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,264,388 | 48,264,409 | GAGGTACTCACAACTGCTCCTG |
| TUP | 48,264,411 | 48,264,432 | TCCCTGTGCAGCTGGTGTTCCT |
| TUR | 48,264,459 | 48,264,479 | ATCCCACCTCTCAAACCCTTG |
| TDF | 48,274,462 | 48,274,484 | CAGGGTATGAGAAGAAGGCTAAC |
| TDP | 48,274,485 | 48,274,510 | TGGTACTAAGGCAGAACAGGCCAAAC |
| TDR | 48,274,516 | 48,274,535 | GCCTGATGGCTTCGGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTGAAGAGTGAGGGCCAGAG |
| SD | GGTGAGCTTGTGAACGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
