12668 Nrip3 Status: Germline transmission achieved
| Gene | Nrip3 |
| Chromosome | 7 |
| Deletion Start | 109,781,470 |
| Deletion End | 109,761,082 |
| Deletion Size | 20,389 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,781,221 | 109,781,241 | GGCCCTTACTGTCACTTTCAC |
| TUP | 109,781,198 | 109,781,220 | TGAGTCACCGACCAGGCTTGTCG |
| TUR | 109,781,178 | 109,781,197 | ACCCGCCAGGGAGTGGATAG |
| TDF | 109,761,365 | 109,761,389 | GGTGAGTTGGGAATAAGGCATTGAG |
| TDP | 109,761,335 | 109,761,357 | TTTAGCATGTAGCCCAGGCTGGC |
| TDR | 109,761,301 | 109,761,320 | TAGGGAGCTGAGGCAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGACAGGTGAGCTGAGAG |
| SD | GGAGAAGTAGAACCTGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
