12680 Cpne7 Status: Cancelled
| Gene | Cpne7 |
| Chromosome | 8 |
| Deletion Start | 123,117,500 |
| Deletion End | 123,134,601 |
| Deletion Size | 17,102 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,117,984 | 123,118,005 | TCACAGGGTCCCACCTTTATTG |
| TUP | 123,118,007 | 123,118,030 | CTGCAGCAGCCATGAGCTTCTCGC |
| TUR | 123,118,050 | 123,118,068 | AGCTCGCAGCCCTAACATC |
| TDF | 123,133,694 | 123,133,714 | TGCCCATGTCTGTCATCATTG |
| TDP | 123,133,721 | 123,133,747 | TGGGCAACGCTGACTTCACAGATATGC |
| TDR | 123,133,762 | 123,133,782 | GGAACGCAGGACTCCATCATC |
PCR Primers
| Primer | Sequence |
| SU | CGATGCCTAGGTTCGATCAG |
| SD | ATGGCAAGATTGTCCAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
