12683 C78339 Status: ES cell colonies screened / QC no positives
| Gene | C78339 |
| Chromosome | 13 |
| Deletion Start | 46,669,589 |
| Deletion End | 46,675,660 |
| Deletion Size | 6,072 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,670,352 | 46,670,374 | TCACTGCCATTCCTACCACTAAC |
| TUP | 46,670,378 | 46,670,403 | TTGGAAGGATCTCACCCTGTGGTGTA |
| TUR | 46,670,410 | 46,670,429 | AGCCTGGCCTACATACTGTG |
| TDF | 46,674,756 | 46,674,774 | TCTCTGGCACTGTGGACTG |
| TDP | 46,674,781 | 46,674,800 | TGGCCTCCAGCCAGTACCCA |
| TDR | 46,674,835 | 46,674,853 | GCTGCTCACTGCTCACTGG |
PCR Primers
| Primer | Sequence |
| SU | ATCGCACACGTGGTTGTGCG |
| SD | TTCTCAGTCACTCTAGGATAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
