12686   Clic6     Status: ES cell colonies screened / QC positives

GeneClic6
Chromosome16
Deletion Start92,498,702
Deletion End92,528,410
Deletion Size29,709
Genome Build37
MGI IDMGI:2146607
NCBI Gene ID209195
BAC ID384C2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGGAGGCCAAATCCACCGAAC
TUPAGGAGGAATCCCAGGCAGAGCTCA
TURTGGGCTCCTCAGCTAAGTG
TDFCGCCTTCATCCTTTGCTGTG
TDPTCTGACTATGCCCAAACCTGCACCAG
TDRCGGAAGCCTGTCCTACACTC

PCR Primers

PrimerSequence
SUGGCTGTCATTGGCTAGCTTC
SDATGATCGGTGGCTGCCTAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12686A-A12VGB6C57BL/6NTac
12686A-B12VGB6C57BL/6NTac
12686A-C9VGB6C57BL/6NTac
12686A-G1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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