12688 Abhd3 Status: Cancelled
| Gene | Abhd3 |
| Chromosome | 18 |
| Deletion Start | 10,706,688 |
| Deletion End | 10,704,555 |
| Deletion Size | 2,134 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,706,117 | 10,706,136 | GTTTCAGCCGCTTCCTACAG |
| TUP | 10,706,083 | 10,706,110 | TGTCCCGTGGTGACAGAAACCTATTACC |
| TUR | 10,706,032 | 10,706,051 | TGAAGGGTCTCAGCAATGTC |
| TDF | 10,704,724 | 10,704,742 | TGTCACTTGCAGCGAACTC |
| TDP | 10,704,690 | 10,704,717 | ACTGCAGACGGAGGACAGATCTCACTGG |
| TDR | 10,704,643 | 10,704,661 | GGTGCTGGCATCCACATAG |
PCR Primers
| Primer | Sequence |
| SU | TTGGATTCTGGAGCAAGTGC |
| SD | AAGTAATAGCTTAGGAGATTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
