12689 C030039L03Rik Status: ES cell colonies screened / QC one positive
| Gene | C030039L03Rik |
| Chromosome | 7 |
| Deletion Start | 27,692,505 |
| Deletion End | 27,704,285 |
| Deletion Size | 11,781 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,692,857 | 27,692,874 | TGCCCTGGGAAATCTGAC |
| TUP | 27,692,880 | 27,692,906 | TCTGAGGTGTTGTCAGGCTGATGACCC |
| TUR | 27,693,011 | 27,693,032 | GCAATCACCTCCCTATCACAGA |
| TDF | 27,703,977 | 27,703,998 | TCAGTGCAAGGAATGTGGAAAG |
| TDP | 27,704,003 | 27,704,032 | TTGTTCGTGGTACAGCTCTTAGAATCCACC |
| TDR | 27,704,041 | 27,704,060 | GGAAGGGCCTCACCAGTATG |
PCR Primers
| Primer | Sequence |
| SU | TGAGATCTGCGCACAGTAGG |
| SD | CCTAAGTAGGAAACAAGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 176 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
