12692 Asb7 Status: ES cell colonies screened / QC no positives
| Gene | Asb7 |
| Chromosome | 7 |
| Deletion Start | 66,679,287 |
| Deletion End | 66,647,707 |
| Deletion Size | 31,581 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,679,038 | 66,679,055 | AAGCTGGGCTGGCGTTTG |
| TUP | 66,679,006 | 66,679,030 | TGCACTTGTGCACGTTTGCATGTGG |
| TUR | 66,678,955 | 66,678,975 | TGCGAAGAGAAGACAAGACAC |
| TDF | 66,648,253 | 66,648,271 | GTGCTCTGCTCTCGTTGAC |
| TDP | 66,648,225 | 66,648,249 | TGTCCTCTAGCTATGTCCCACCTCC |
| TDR | 66,648,202 | 66,648,220 | TGGTGAGTGCTGTGGAATC |
PCR Primers
| Primer | Sequence |
| SU | CTTAGCTTCCTGATGCCACC |
| SD | ATCACCCAGGATCACAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
