12695 Cys1 Status: ES cell colonies screened / QC no positives
| Gene | Cys1 |
| Chromosome | 12 |
| Deletion Start | 24,680,905 |
| Deletion End | 24,667,080 |
| Deletion Size | 13,826 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,680,311 | 24,680,329 | GCTGCACGGAGTCGAGTTG |
| TUP | 24,680,287 | 24,680,310 | CAGCATCCTTACAGCCCAGGTGGG |
| TUR | 24,680,232 | 24,680,250 | GCCTCCTCCCAGCGTATTC |
| TDF | 24,667,997 | 24,668,018 | GGAAGGGCTTGTTTCTGTTGAG |
| TDP | 24,667,971 | 24,667,994 | TCTCCAGACTGGCAGGAAAGCCAA |
| TDR | 24,667,952 | 24,667,970 | CAGGGAGCTGTGCAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | TAGCAGCCTCTACTTCCTCC |
| SD | CGGAGGAAACTGCATCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
