12700 Fut10 Status: ES cell colonies screened / QC no positives
| Gene | Fut10 |
| Chromosome | 8 |
| Deletion Start | 31,195,212 |
| Deletion End | 31,201,498 |
| Deletion Size | 6,287 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,195,629 | 31,195,649 | GTGAAGGGTGGTCTGGAACTC |
| TUP | 31,195,655 | 31,195,684 | ACTTCCGCTTCTAGCTCCTAAGTATTGGGA |
| TUR | 31,195,698 | 31,195,716 | CGACTGGGCATGGTGGTAG |
| TDF | 31,200,955 | 31,200,979 | GATGTCAACTGAGGAGGAAGGAAAG |
| TDP | 31,200,981 | 31,201,006 | TGGGTGCTTTCTCTGTCTCTAGGAGC |
| TDR | 31,201,014 | 31,201,036 | GGAGCCAAATGCTAGTGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGTAAGGCTATGCCTGTC |
| SD | CAGGGTTGTTTGATATCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
