12704 Ces2c Status: Cancelled
| Gene | Ces2c |
| Chromosome | 8 |
| Deletion Start | 104,847,136 |
| Deletion End | 104,854,357 |
| Deletion Size | 7,222 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,847,400 | 104,847,424 | TCCTTACTGTAATGTTGCTCTTGAG |
| TUP | 104,847,425 | 104,847,453 | TGGCTTCTCAGTTCCTAAGATCATCAGGA |
| TUR | 104,847,460 | 104,847,480 | GGGCCATGACCATTGGAAATC |
| TDF | 104,853,637 | 104,853,659 | CTTTGCACGACATGGGTGAGAAT |
| TDP | 104,853,660 | 104,853,685 | ACACCCCTCAACCTAACGTGTGTTGG |
| TDR | 104,853,702 | 104,853,724 | CTACAAGGCCACCATCCAATAGG |
PCR Primers
| Primer | Sequence |
| SU | AAGCACTCTGGCTTCTCCTG |
| SD | CATTCCAAACTCCTGAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
