12707 Gldn Status: ES cell colonies screened / QC one positive
Gene | Gldn |
Chromosome | 9 |
Deletion Start | 54,286,527 |
Deletion End | 54,312,938 |
Deletion Size | 26,412 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 54,287,077 | 54,287,098 | GCAAGCCTTCACTTCTGACATC |
TUP | 54,287,100 | 54,287,127 | TTTGAGTCTGCAGGTAAATGAGGGACGT |
TUR | 54,287,165 | 54,287,185 | GTCAGTTCACACCCAGGAAAC |
TDF | 54,312,318 | 54,312,340 | TGAACTCACTCTGTAGCCCAAAC |
TDP | 54,312,343 | 54,312,369 | AGCCTTGAACTTGCCATCCTTTCGCCT |
TDR | 54,312,376 | 54,312,396 | CAGTAATCCCAGCCCTTCCAC |
PCR Primers
Primer | Sequence |
SU | GGCTTTAAGAATTCCTGCCC |
SD | CAATGCTGCACATCTGATGC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 109 bp | + | + | - |
TDF | TDR | 79 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |