12709 Pgbd5 Status: Cancelled
| Gene | Pgbd5 |
| Chromosome | 8 |
| Deletion Start | 124,384,605 |
| Deletion End | 124,370,474 |
| Deletion Size | 14,132 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,384,543 | 124,384,562 | CGGACAACGTGCTCAAGAAC |
| TUP | 124,384,515 | 124,384,541 | TGGTGGTACAGACCAACATGTATGCCA |
| TUR | 124,384,481 | 124,384,498 | GCACCGTCACTGCCAAAC |
| TDF | 124,370,641 | 124,370,659 | GACGTGGCAGCAAGTGTTC |
| TDP | 124,370,620 | 124,370,640 | TGGTTCGCCATCAGCATCGCC |
| TDR | 124,370,554 | 124,370,572 | TGCCCGGCTGTACTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AACAGAACAGCAGGATTGGG |
| SD | TGCATCTCTTCTTCCAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
