12711   C530028O21Rik     Status: ES cell colonies screened / QC no positives

GeneC530028O21Rik
Chromosome6
Deletion Start124,998,976
Deletion End125,002,032
Deletion Size3,057
Genome Build38
MGI IDMGI:2441908
NCBI Gene ID319352
BAC ID114M21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF124,999,806124,999,828TCTGCGGAAATTGAGGCTTTATC
TUP124,999,831124,999,854TGAGTGTCTCCAGCAAATGTGCCA
TUR124,999,868124,999,890CAGCTTCACCTCTGCATCCTTTG
TDF125,001,132125,001,154GCTTGAACTAAATCTGCCAGGTC
TDP125,001,156125,001,178CCGCAGAGGAGAGCAAGGCTCCT
TDR125,001,234125,001,253AGGGCCACAGGCTTAATGAC

PCR Primers

PrimerSequence
SUAAGGAAGGAAGCCAAAGAGG
SDACATAGGCAACACGAGGGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR85 bp++-
TDFTDR122 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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