12712 6430573F11Rik Status: ES cell colonies screened / QC no positives
| Gene | 6430573F11Rik |
| Chromosome | 8 |
| Deletion Start | 36,498,331 |
| Deletion End | 36,512,661 |
| Deletion Size | 14,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,498,942 | 36,498,965 | ATCCACACCAACCTGACACAGATG |
| TUP | 36,498,975 | 36,499,002 | AAGGATGGAAACGCCATGACGTTAGACG |
| TUR | 36,499,043 | 36,499,065 | CACTCACCATGAGCCAAGGATAC |
| TDF | 36,511,848 | 36,511,869 | AAGGGCAGATGTGATTCCAAAC |
| TDP | 36,511,873 | 36,511,897 | CCCACAGCATGGACTATGGGTCTGC |
| TDR | 36,511,906 | 36,511,923 | TAGCTTCGCAGCAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATTGATGCTTCATGAAGGTTTC |
| SD | AGTCTCAAACACACCCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
