12713 4732471D19Rik Status: Cancelled
| Gene | 4732471D19Rik |
| Chromosome | 13 |
| Deletion Start | 54,525,341 |
| Deletion End | 54,550,681 |
| Deletion Size | 25,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,525,515 | 54,525,541 | CCAGGAGATGTGATACAGTCACTAAAC |
| TUP | 54,525,546 | 54,525,573 | TGCCACAGTCATCAAGAGATAGGATGCA |
| TUR | 54,525,584 | 54,525,603 | CCTGATGCTGATGGCACATG |
| TDF | 54,549,843 | 54,549,864 | TGGATTGAGGTGCTAGGAAGTG |
| TDP | 54,549,866 | 54,549,889 | AGCCACACCTCCTCGTGCTCAACA |
| TDR | 54,549,919 | 54,549,937 | GTGTGGTGGCGCAAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TACCCATATCAAGACACTCTG |
| SD | CAGGCTCTTGTTTCTTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
