| Gene | Sidt1 |
| Chromosome | 16 |
| Deletion Start | 44,332,525 |
| Deletion End | 44,299,808 |
| Deletion Size | 32,718 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,331,905 | 44,331,928 | GGACCCTACTATTTCATGGCACTC |
| TUP | 44,331,877 | 44,331,904 | CAGGAAGCAGAGGTGCTCAGAAATGGAA |
| TUR | 44,331,850 | 44,331,869 | GCAGAACCCTGCTCACTCTC |
| TDF | 44,300,007 | 44,300,027 | TGTGTTCACTGTGTCTCCTTC |
| TDP | 44,299,977 | 44,300,004 | CTTGCTGATGTTGTCTATACCCGCGGCT |
| TDR | 44,299,950 | 44,299,973 | GGTAGTTGTAGCTCCTTTGGTATC |
PCR Primers
| Primer | Sequence |
| SU | CGATTCTTCCAGACTAGCGG |
| SD | ATGTCATTCGTTACCTTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12716A-D6 | VGB6 | C57BL/6NTac | |
| 12716A-E3 | VGB6 | C57BL/6NTac | |
